Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED.

ABSTRACT: Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation of large, imperfect crystals into microcrystals or nanocrystals can provide a simple path for high-resolution structure determination by the cryoEM method MicroED and potentially by serial femtosecond crystallography.

SUBMITTER: de la Cruz MJ 

PROVIDER: S-EPMC5376236 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature