Intraoperative Near-Infrared Fluorescence Imaging of Thymus in Preclinical Models.

ABSTRACT:

Background

There are currently no thymus-specific contrast agents for biomedical imaging. Thus, finding ectopic thymic tissue during certain operations is extremely difficult. The purpose of the present study was to determine if near-infrared (NIR) fluorescence imaging could provide high sensitivity, real-time identification of thymic tissue during the operation.

Methods

After initial in vivo screening of a 315-compound NIR fluorophore library for thymic uptake, methylene blue and five different 700-nm emitting candidate molecules were injected into CD-1 mice for quantitation of the signal-to-background ratio as a function of kinetics and dosing. Results were confirmed in 35-kg Yorkshire pigs. Dual-channel NIR imaging was also performed using a variety of 800-nm emitting NIR

SUBMITTER: Wada H 

PROVIDER: S-EPMC5376245 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature