Proteomic analysis of protein purified derivative of Mycobacterium bovis.

ABSTRACT:

Background

Tuberculin skin test based on in vivo intradermal inoculation of purified protein derivative from Mycobacterium bovis (bPPD) is the diagnostic test for the control and surveillance of bovine tuberculosis (bTB).

Methods

Proteomic analysis was performed on different bPPD preparations from M. bovis, strain AN5. Proteins were precipitated from bPPD solutions by TCA precipitation. The proteome of bPPD preparations was investigated by bottom-up proteomics, which consisted in protein digestion and nano-LC-MS/MS analysis. Mass spectrometry analysis was performed on a Q-exactive hybrid quadrupole-Orbitrap mass spectrometer coupled online to an Easy nano-LC1000 system.

Results

Three hundred and fifty-six proteins were identified and quantified by at least 2 peptides

SUBMITTER: Roperto S 

PROVIDER: S-EPMC5376687 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature