Id2 controls specification of Lgr5⁺ intestinal stem cell progenitors during gut development.

ABSTRACT: The adult intestinal stem cells (ISCs), their hierarchies, mechanisms of maintenance and differentiation have been extensively studied. However, when and how ISCs are established during embryogenesis remains unknown. We show here that the transcription regulator Id2 controls the specification of embryonic Lgr5⁺ progenitors in the developing murine small intestine. Cell fate mapping analysis revealed that Lgr5⁺ progenitors emerge at E13.5 in wild-type embryos and differ from the rest on the intestinal epithelium by a characteristic ISC signature. In the absence of Id2, the intestinal epithelium differentiates into Lgr5⁺ cells already at E9.5. Furthermore, the size of the Lgr5⁺ cell pool is significantly increased. We show that Id2 restricts the ac

SUBMITTER: Nigmatullina L 

PROVIDER: S-EPMC5376969 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature