Automated pipeline for purification, biophysical and x-ray analysis of biomacromolecular solutions.

ABSTRACT: Small angle X-ray scattering (SAXS), an increasingly popular method for structural analysis of biological macromolecules in solution, is often hampered by inherent sample polydispersity. We developed an all-in-one system combining in-line sample component separation with parallel biophysical and SAXS characterization of the separated components. The system coupled to an automated data analysis pipeline provides a novel tool to study difficult samples at the P12 synchrotron beamline (PETRA-3, EMBL/DESY, Hamburg).

SUBMITTER: Graewert MA 

PROVIDER: S-EPMC5377070 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature