A sensitive method for the quantification of virion-sense and complementary-sense DNA strands of circular single-stranded DNA viruses.

ABSTRACT: Circular single-stranded DNA (ssDNA) viruses are the smallest viruses known to infect eukaryotes. High recombination and mutation rates have conferred these viruses with an evolutionary potential that has facilitated their emergence. Their damaging effects on livestock (circoviruses) and crops (geminiviruses and nanoviruses), and the ubiquity of anelloviruses in human populations and other mammalian species, have resulted in increased interest in better understanding their epidemiology and infection mechanisms. Circular ssDNA viral replication involves the synthesis of dsDNA intermediates containing complementary-sense (CS) and virion-sense (VS) strands. Precise quantification of VS and CS accumulation during viral infections can provide insights into the molecular mechanisms underlying vi

SUBMITTER: Rodriguez-Negrete EA 

PROVIDER: S-EPMC5377365 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature