A Model for Designing Adaptive Laboratory Evolution Experiments.

ABSTRACT: The occurrence of mutations is a cornerstone of the evolutionary theory of adaptation, capitalizing on the rare chance that a mutation confers a fitness benefit. Natural selection is increasingly being leveraged in laboratory settings for industrial and basic science applications. Despite increasing deployment, there are no standardized procedures available for designing and performing adaptive laboratory evolution (ALE) experiments. Thus, there is a need to optimize the experimental design, specifically for determining when to consider an experiment complete and for balancing outcomes with available resources (i.e., laboratory supplies, personnel, and time). To design and to better understand ALE experiments, a simulator, ALEsim, was developed, validated, and applied to the optimization o

SUBMITTER: LaCroix RA 

PROVIDER: S-EPMC5377496 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature