Revisiting the Regulation of the Primary Scaffoldin Gene in Clostridium thermocellum.

ABSTRACT: Cellulosomes are considered to be one of the most efficient systems for the degradation of plant cell wall polysaccharides. The central cellulosome component comprises a large, noncatalytic protein subunit called scaffoldin. Multiple saccharolytic enzymes are incorporated into the scaffoldins via specific high-affinity cohesin-dockerin interactions. Recently, the regulation of genes encoding certain cellulosomal components by multiple RNA polymerase alternative σ^I factors has been demonstrated in Clostridium (Ruminiclostridium) thermocellum In the present report, we provide experimental evidence demonstrating that the C. thermocellum cipA gene, which encodes the primary cellulosomal scaffoldin, is regulated by several alternative σ^I factors

SUBMITTER: Ortiz de Ora L 

PROVIDER: S-EPMC5377506 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature