Catalytic Determinants of Alkene Production by the Cytochrome P450 Peroxygenase OleT_JE.

ABSTRACT: The Jeotgalicoccus sp. peroxygenase cytochrome P450 OleT_JE (CYP152L1) is a hydrogen peroxide-driven oxidase that catalyzes oxidative decarboxylation of fatty acids, producing terminal alkenes with applications as fine chemicals and biofuels. Understanding mechanisms that favor decarboxylation over fatty acid hydroxylation in OleT_JE could enable protein engineering to improve catalysis or to introduce decarboxylation activity into P450s with different substrate preferences. In this manuscript, we have focused on OleT_JE active site residues Phe⁷⁹, His⁸⁵, and Arg²⁴⁵ to interrogate their roles in substrate binding and catalytic activity. His⁸⁵ is a potential proton donor to reactive iron-oxo species during subs

SUBMITTER: Matthews S 

PROVIDER: S-EPMC5377825 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature