Infection by Rhodococcus fascians maintains cotyledons as a sink tissue for the pathogen.

ABSTRACT:

Background and aims

Pisum sativum L. (pea) seed is a source of carbohydrate and protein for the developing plant. By studying pea seeds inoculated by the cytokinin-producing bacterium, Rhodococcus fascians , we sought to determine the impact of both an epiphytic (avirulent) strain and a pathogenic strain on source-sink activity within the cotyledons during and following germination.

Methods

Bacterial spread was monitored microscopically, and real-time reverse transcription-quantitative PCR was used to determine the expression of cytokinin biosynthesis, degradation and response regulator gene family members, along with expression of family members of SWEET , SUT , CWINV and AAP genes - gene families identified initially in pea by transcriptomic analysis. The endogenous cytoki

SUBMITTER: Dhandapani P 

PROVIDER: S-EPMC5378184 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature