Hypoxia promotes the phenotypic change of aldehyde dehydrogenase activity of breast cancer stem cells.

ABSTRACT: Stable breast cancer cell (BCC) lines are valuable tools for the identification of breast cancer stem cell (BCSC) phenotypes that develop in response to several stimuli as well as for studying the basic mechanisms associated with the initiation and maintenance of BCSCs. However, the characteristics of individual, BCC-derived BCSCs varies and these cells show distinct phenotypes depending on the different BCSC markers used for their isolation. Aldehyde dehydrogenase (ALDH) activity is just such a recognized biomarker of BCSCs with a CD44⁺ /CD24^- phenotype. We isolated BCSCs with high ALDH activity (CD44⁺ /CD24^- /Aldefluor^pos ) from a primary culture of human breast cancer tissue and observed that the cells had stem cell properties compa

SUBMITTER: Shiraishi A 

PROVIDER: S-EPMC5378271 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature