Isolation and characterization of nontuberculous mycobacteria from patients with pulmonary tuberculosis in Ghana.

ABSTRACT:

Objective/background

Nontuberculous mycobacterial (NTM) species are assuming public health importance in pulmonary diseases; they are increasingly being isolated, and importantly, most NTMs do not respond to routine tuberculosis (TB) drugs. This study aimed to identify NTMs isolated from pulmonary TB cases and also determine their susceptibility to streptomycin (STR), isoniazid (INH), and rifampicin (RIF).

Methods

A total of 1755 mycobacterial isolates, obtained between August 2012 and July 2014, from 2036 smear-positive pulmonary cases were identified using polymerase chain reaction amplification of IS6110, and hsp65 gene sequencing analysis. Drug susceptibility testing (DST) was then performed for the identified NTMs against STR, INH, and RIF using microplate Alamar blue a

SUBMITTER: Otchere ID 

PROVIDER: S-EPMC5378301 | biostudies-literature | 2017 Jan-Mar

REPOSITORIES: biostudies-literature