Deep Sequencing of 71 Candidate Genes to Characterize Variation Associated with Alcohol Dependence.

ABSTRACT:

Background

Previous genomewide association studies (GWASs) have identified a number of putative risk loci for alcohol dependence (AD). However, only a few loci have replicated and these replicated variants only explain a small proportion of AD risk. Using an innovative approach, the goal of this study was to generate hypotheses about potentially causal variants for AD that can be explored further through functional studies.

Methods

We employed targeted capture of 71 candidate loci and flanking regions followed by next-generation deep sequencing (mean coverage 78X) in 806 European Americans. Regions included in our targeted capture library were genes identified through published GWAS of alcohol, all human alcohol and aldehyde dehydrogenases, reward system genes including dopa

SUBMITTER: Clark SL 

PROVIDER: S-EPMC5378639 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature