Outlining Core Pathways of Amyloid Toxicity in Bacteria with the RepA-WH1 Prionoid.

ABSTRACT: The synthetic bacterial prionoid RepA-WH1 causes a vertically transmissible amyloid proteinopathy in Escherichia coli that inhibits growth and eventually kills the cells. Recent in vitro studies show that RepA-WH1 builds pores through model lipid membranes, suggesting a possible mechanism for bacterial cell death. By comparing acutely (A31V) and mildly (ΔN37) cytotoxic mutant variants of the protein, we report here that RepA-WH1(A31V) expression decreases the intracellular osmotic pressure and compromise bacterial viability under either aerobic or anaerobic conditions. Both are effects expected from threatening membrane integrity and are in agreement with findings on the impairment by RepA-WH1(A31V) of the proton motive force (PMF)-dependent transport of ions (Fe³⁺

SUBMITTER: Molina-Garcia L 

PROVIDER: S-EPMC5378768 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature