Photodynamic Physiology-Photonanomanipulations in Cellular Physiology with Protein Photosensitizers.

ABSTRACT: Singlet oxygen generated in a type II photodynamic action, due to its limited lifetime (1 μs) and reactive distance (<10 nm), could regulate live cell function nanoscopically. The genetically-encoded protein photosensitizers (engineered fluorescent proteins such as KillerRed, TagRFP, and flavin-binding proteins such as miniSOG, Pp2FbFP^L30M) could be expressed in a cell type- and/or subcellular organelle-specific manner for targeted protein photo-oxidative activation/desensitization. The newly emerged active illumination technique provides an additional level of specificity. Typical examples of photodynamic activation include permanent activation of G protein-coupled receptor CCK1 and photodynamic activation of ionic channel TRPA1. Protein photosensitizers have been used t

SUBMITTER: Jiang HN 

PROVIDER: S-EPMC5378799 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature