Predominant structural configuration of natural antibody repertoires enables potent antibody responses against protein antigens.

ABSTRACT: Humoral immunity against diverse pathogens is rapidly elicited from natural antibody repertoires of limited complexity. But the organizing principles underlying the antibody repertoires that facilitate this immunity are not well-understood. We used HER2 as a model immunogen and reverse-engineered murine antibody response through constructing an artificial antibody library encoded with rudimentary sequence and structural characteristics learned from high throughput sequencing of antibody variable domains. Antibodies selected in vitro from the phage-displayed synthetic antibody library bound to the model immunogen with high affinity and specificities, which reproduced the specificities of natural antibody responses. We conclude that natural antibody structural repertoires are shaped to allow

SUBMITTER: Chen HS 

PROVIDER: S-EPMC5378893 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature