Localization of Cl-35 nuclei in biological solids using rotational-echo double-resonance experiments.

ABSTRACT: Chloride ions play important roles in many chemical and biological processes. This paper investigates the possibility of localizing ³⁵Cl nuclei using solid-state NMR. It demonstrates that distances shorter than 3.8Å, between ¹³C atoms and ³⁵Cl atoms in 10% uniformly labeled ¹³C L-tyrosine·HCl and natural abundance Glycine·HCl can be measured using rotational-echo (adiabatic passage) double-resonance (RE(AP)DOR). Furthermore the effect of quadrupolar interaction on the REDOR/REAPDOR experiment is quantified. The dephasing curve is plotted in a three dimensional chart as a function of the dephasing time and of the strength of quadrupolar interaction felt by each orientation. During spinning each orientation feels a quadrupolar interaction that vari

SUBMITTER: Iuga D 

PROVIDER: S-EPMC5378917 | biostudies-literature | 2017 Apr - May

REPOSITORIES: biostudies-literature