A fluorescence-quenching platform based on biomineralized hydroxyapatite from natural seashell and applied to cancer cell detection.

ABSTRACT: As a typical biomineral, hydroxyapatite (HAp) is widely applied in bone implants and other related fields. However, the inherent nature of HAp can potentially be altered through restricting its fabrication conditions. Here, HAp fabricated by a hydrothermal treatment of pieces of natural seashell is demonstrated to have the capability of fluorescence quenching. To the best of the author's knowledge, this is the first time that this new property of HAp has been reported. Consequently, we assembled a fluorescence-quenching platform based on the biomineralized HAp substrate following a hydrothermal treatment and associated with a DNA molecular beacon and applied to cancer cell detection by the transformation from "OFF state" (fluorescence quenching) to "ON state" (fluorescence recovery). Herei

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC5378986 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature