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XTEND: Extending the depth of field in cryo soft X-ray tomography.

ABSTRACT: We have developed a new data collection method and processing framework in full field cryo soft X-ray tomography to computationally extend the depth of field (DOF) of a Fresnel zone plate lens. Structural features of 3D-reconstructed eukaryotic cells that are affected by DOF artifacts in standard reconstruction are now recovered. This approach, based on focal series projections, is easily applicable with closed expressions to select specific data acquisition parameters.

SUBMITTER: Oton J

PROVIDER: S-EPMC5379191 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

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XTEND: Extending the depth of field in cryo soft X-ray tomography.

Otón Joaquín J Pereiro Eva E Conesa José J JJ Chichón Francisco J FJ Luque Daniel D Rodríguez Javier M JM Pérez-Berná Ana J AJ Sorzano Carlos Oscar S CO Klukowska Joanna J Herman Gabor T GT Vargas Javier J Marabini Roberto R Carrascosa José L JL Carazo José M JM

Scientific reports 20170404

We have developed a new data collection method and processing framework in full field cryo soft X-ray tomography to computationally extend the depth of field (DOF) of a Fresnel zone plate lens. Structural features of 3D-reconstructed eukaryotic cells that are affected by DOF artifacts in standard reconstruction are now recovered. This approach, based on focal series projections, is easily applicable with closed expressions to select specific data acquisition parameters. ...[more]

PMID: 28374769

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