Comparison of triple-negative breast cancer molecular subtyping using RNA from matched fresh-frozen versus formalin-fixed paraffin-embedded tissue.

ABSTRACT:

Background

Triple negative breast cancer (TNBC) is a heterogeneous disease that lacks unifying molecular alterations that can guide therapy decisions. We previously identified distinct molecular subtypes of TNBC (TNBCtype) using gene expression data generated on a microarray platform using frozen tumor specimens. Tumors and cell lines representing the identified subtypes have distinct enrichment in biologically relevant transcripts with differing sensitivity to standard chemotherapies and targeted agents. Since our initial discoveries, RNA-sequencing (RNA-seq) has evolved as a sensitive and quantitative tool to measure transcript abundance.

Methods

To demonstrate that TNBC subtypes were similar between platforms, we compared gene expression from matched specimens profiled by

SUBMITTER: Jovanovic B 

PROVIDER: S-EPMC5379658 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature