Polynucleotide phosphorylase is implicated in homologous recombination and DNA repair in Escherichia coli.

ABSTRACT:

Background

Polynucleotide phosphorylase (PNPase, encoded by pnp) is generally thought of as an enzyme dedicated to RNA metabolism. The pleiotropic effects of PNPase deficiency is imputed to altered processing and turnover of mRNAs and small RNAs, which in turn leads to aberrant gene expression. However, it has long since been known that this enzyme may also catalyze template-independent polymerization of dNDPs into ssDNA and the reverse phosphorolytic reaction. Recently, PNPase has been implicated in DNA recombination, repair, mutagenesis and resistance to genotoxic agents in diverse bacterial species, raising the possibility that PNPase may directly, rather than through control of gene expression, participate in these processes.

Results

In this work we present evidence that

SUBMITTER: Carzaniga T 

PROVIDER: S-EPMC5379764 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature