Fission yeast myosin I facilitates PI(4,5)P₂-mediated anchoring of cytoplasmic dynein to the cortex.

ABSTRACT: Several key processes in the cell, such as vesicle transport and spindle positioning, are mediated by the motor protein cytoplasmic dynein, which produces force on the microtubule. For the functions that require movement of the centrosome and the associated nuclear material, dynein needs to have a stable attachment at the cell cortex. In fission yeast, Mcp5 is the anchor protein of dynein and is required for the oscillations of the horsetail nucleus during meiotic prophase. Although the role of Mcp5 in anchoring dynein to the cortex has been identified, it is unknown how Mcp5 associates with the membrane as well as the importance of the underlying attachment to the nuclear oscillations. Here, we set out to quantify Mcp5 organization and identify the binding partner of Mcp5 at the membrane.

SUBMITTER: Thankachan JM 

PROVIDER: S-EPMC5380020 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature