Large-scale identification of coevolution signals across homo-oligomeric protein interfaces by direct coupling analysis.

ABSTRACT: Proteins have evolved to perform diverse cellular functions, from serving as reaction catalysts to coordinating cellular propagation and development. Frequently, proteins do not exert their full potential as monomers but rather undergo concerted interactions as either homo-oligomers or with other proteins as hetero-oligomers. The experimental study of such protein complexes and interactions has been arduous. Theoretical structure prediction methods are an attractive alternative. Here, we investigate homo-oligomeric interfaces by tracing residue coevolution via the global statistical direct coupling analysis (DCA). DCA can accurately infer spatial adjacencies between residues. These adjacencies can be included as constraints in structure prediction techniques to predict high-resolution mode

SUBMITTER: Uguzzoni G 

PROVIDER: S-EPMC5380090 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature