Evaluating normalization approaches for the better identification of aberrant microRNAs associated with hepatocellular carcinoma.

ABSTRACT:

Aim

Dysregulated microRNAs (miRNAs) have been identified in hepatocellular carcinoma (HCC), but only a small proportion have been confirmed. An appropriate normalizer is crucial to determining the accuracy and reliability of data from miRNA studies.

Methods

Different normalization strategies were used to validate genome-wide miRNA profiles in HCC tumor and non-tumor tissues, and to determine the consistency and discrepancy of data on dysregulated miRNAs.

Results

Two sets of stable miRNAs (miR-30c/miR-30b and miR-30c/miR-126) were identified in HCC tissues by geNorm and NormFinder tools, respectively. The mean of global miRNAs also showed good stability for ranking the top 1-2 miRNAs, but the stabilities of the manufacturer-recommended ncRNAs controls were poor. Four p

SUBMITTER: Shen J 

PROVIDER: S-EPMC5380371 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature