Tracking dendritic cell migration into lymph nodes by using a novel PET probe ¹⁸F-tetrafluoroborate for sodium/iodide symporter.

ABSTRACT:

Background

Recently, ¹⁸F-tetrafluoroborate (TFB) was used as a substrate for the human sodium/iodide symporter (hNIS) reporter gene. This study evaluated the feasibility of performing molecular-genetic imaging by using the new radiotracer (¹⁸F-TFB) for the hNIS gene, to track dendritic cell (DC) migration in live mice. A murine dendritic cell line (DC2.4) co-expressing the hNIS and effluc genes (DC/NF) was established. To confirm the functional cellular expression of both effluc and NIS in the inoculated DC/NF cells by bio-medical imaging, combined bioluminescence imaging (BLI) and ¹⁸F-TFB positron emission tomography/computed tomography (PET/CT) imaging was performed after intramuscular injection with parental DCs and DC/NF cells. For DC-tracking,

SUBMITTER: Lee SB 

PROVIDER: S-EPMC5380646 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature