A novel scalable, robust downstream process for oncolytic rat parvovirus: isoelectric point-based elimination of empty particles.

ABSTRACT: The rodent protoparvovirus H-1PV, with its oncolytic and oncosuppressive properties, is a promising anticancer agent currently under testing in clinical trials. This explains the current demand for a scalable, good manufacturing practice-compatible virus purification process yielding high-grade pure infectious particles and overcoming the limitations of the current system based on density gradient centrifugation. We describe here a scalable process offering high purity and recovery. Taking advantage of the isoelectric point difference between full and empty particles, it eliminates most empty particles. Full particles have a significantly higher cationic charge than empty ones, with an isoelectric point of 5.8-6.2 versus 6.3 (as determined by isoelectric focusing and chromatofocusing). Tha

SUBMITTER: Leuchs B 

PROVIDER: S-EPMC5380694 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature