Effect of Cell Seeding Density and Inflammatory Cytokines on Adipose Tissue-Derived Stem Cells: an in Vitro Study.

ABSTRACT: Adipose tissue-derived stem cells (ASCs) are known to be able to promote repair of injured tissue via paracrine factors. However, the effect of cell density and inflammatory cytokines on the paracrine ability of ASCs remains largely unknown. To investigate these effects, ASCs were cultured in 8000 cells/cm², 20,000 cells/cm², 50,000 cells/cm², and 400,000 cells/cm² with and without 10 or 20 ng/ml tumor necrosis factor alpha (TNFα) and 25 or 50 ng/ml interferon gamma (IFNγ). ASC-sheets formed at 400,000 cells/cm² after 48 h of culture. With increasing concentrations of TNFα and IFNγ, ASC-sheets with 400,000 cells/cm² had increased production of angiogenic factors Vascular Endothelial Growth Factor and Fibroblast Growth Factor

SUBMITTER: Sukho P 

PROVIDER: S-EPMC5380713 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature