DNA-barcoded labeling probes for highly multiplexed Exchange-PAINT imaging.

ABSTRACT: Recent advances in super-resolution fluorescence imaging allow researchers to overcome the classical diffraction limit of light, and are already starting to make an impact in biology. However, a key challenge for traditional super-resolution methods is their limited multiplexing capability, which prevents a systematic understanding of multi-protein interactions on the nanoscale. Exchange-PAINT, a recently developed DNA-based multiplexing approach, in theory facilitates spectrally-unlimited multiplexing by sequentially imaging target molecules using orthogonal dye-labeled 'imager' strands. While this approach holds great promise for the bioimaging community, its widespread application has been hampered by the availability of DNA-conjugated ligands for protein labeling. Herein, we report a u

SUBMITTER: Agasti SS 

PROVIDER: S-EPMC5380918 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature