A novel label-free fluorescence assay for one-step sensitive detection of Hg²⁺ in environmental drinking water samples.

ABSTRACT: A novel label-free fluorescence assay for detection of Hg²⁺ was developed based on the Hg²⁺-binding single-stranded DNA (ssDNA) and SYBR Green I (SG I). Differences from other assays, the designed rich-thymine (T) ssDNA probe without fluorescent labelling can be rapidly formed a T-Hg²⁺-T complex and folded into a stable hairpin structure in the presence of Hg²⁺ in environmental drinking water samples by facilitating fluorescence increase through intercalating with SG I in one-step. In the assay, the fluorescence signal can be directly obtained without additional incubation within 1 min. The dynamic quantitative working ranges was 5-1000 nM, the determination coefficients were satisfied by optimization of the reaction conditions. The lowest detect

SUBMITTER: Li Y 

PROVIDER: S-EPMC5380999 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature