Transcriptional signatures of steroid hormones in the striatal neurons and astrocytes.

ABSTRACT:

Background

The mechanisms of steroids actions in the brain mainly involve the binding and nuclear translocation of specific cytoplasmic receptors. These receptors can act as transcription factors and regulate gene expression. However, steroid-dependent transcriptional regulation in different types of neural cells is not yet fully understood. The aim of this study was to evaluate and compare transcriptional alterations induced by various steroid receptor agonists in primary cultures of astrocytes and neurons from mouse brain.

Results

We utilized whole-genome microarrays (Illumina Mouse WG-6) and quantitative PCR analyses to measure mRNA abundance levels. To stimulate gene expression we treated neuronal and astroglial cultures with dexamethasone (100 nM), aldosterone (200 nM),

SUBMITTER: Piechota M 

PROVIDER: S-EPMC5381047 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature