Detection of newly produced T and B lymphocytes by digital PCR in blood stored dry on nylon flocked swabs.

ABSTRACT:

Background

A normal number of T-cell receptor excision circles (TRECs) and K-deleting recombination excision circles (KRECs) is considered a biomarker for adequate new T- and B-cell production. In newborns, detection of TRECs and KRECs by real time PCR from dried blood spotted on filter paper is used for the screening of severe immunodeficiency. In adults, elderly and during diseases, where the number of TRECs is lower than in newborns and children, a large amount of DNA and a sensitive method of amplification are necessary to identify newly produced lymphocytes.

Methods

DNA was prepared from blood of 203 healthy adults (range: 18-91 years old) absorbed for 10 s on flocked swabs and let to dry, or from peripheral blood mononuclear cells. DNA was subjected to digital PCR and

SUBMITTER: Tessitore MV 

PROVIDER: S-EPMC5381048 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature