Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study.

ABSTRACT:

Background

Indoor air pollution caused by fungal contamination is suspected to have a public health impact. Monitoring of the composition of the indoor airborne fungal contaminants is therefore important. To avoid problems linked to culture-dependent protocols, molecular methods are increasingly being proposed as an alternative. Among these molecular methods, the polymerase chain reaction (PCR) and the real-time PCR are the most frequently used tools for indoor fungal detection. However, even if these tools have demonstrated their appropriate performance, some of them are not able to discriminate between species which are genetically close. A solution to this could be the use of a post-qPCR high resolution melting (HRM) analysis, which would allow the discrimination of these specie

SUBMITTER: Libert X 

PROVIDER: S-EPMC5381056 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature