A Fluorometric Method of Measuring Carboxypeptidase Activities for Angiotensin II and Apelin-13.

ABSTRACT: Degradation of the biologically potent octapeptide angiotensin Ang II-(1-8) is mediated by the activities of several peptidases. The conversion of Ang II to the septapeptide Ang-(1-7) is of particular interest as the latter also confers organ protection. The conversion is catalyzed by angiotensin-converting enzyme 2 and other enzymes that selectively cleave the peptide bond between the proline and the phenylalanine at the carboxyl terminus of Ang II. The contribution of various enzyme activities that collectively lead to the formation of Ang-(1-7) from Ang II, in both normal conditions and in disease states, remains only partially understood. This is largely due to the lack of a reliable and sensitive method to detect these converting activities in complex samples, such as blood and tissue

SUBMITTER: Liu P 

PROVIDER: S-EPMC5381230 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature