Sensitive detection of protein biomarkers using silver nanoparticles enhanced immunofluorescence assay.

ABSTRACT: Detection of biomarkers is extremely important for the early diagnosis of diseases. Here, we developed an easy and highly sensitive fluorescence detection system for the determination of biomarkers by combining the rapid separation of magnetic beads and silver nanoparticles labeled antibodies. An ultrasensitive silver ions fluorescence probe 3', 6'-bis (diethylamino)-2-(2-iodoethyl) spiro[isoindoline-1, 9'-xanthen]-3-one (Ag⁺-FP) was applied to immunoassay. A significant signal amplification was achieved as the AgNPs can be dissolved by H₂O₂ and generate numerous Ag⁺, which would turn "on" the fluorescence of Ag⁺-FP. Using α-fetoprotein (AFP) and C-reactive protein (CRP) as target analytes, good linear responses were obtained from 0.1

SUBMITTER: Zhao LJ 

PROVIDER: S-EPMC5381250 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature