RNAi-nanoparticulate manipulation of gene expression as a new functional genomics tool in the liver.

ABSTRACT:

Background & aims

The Hippo pathway controls organ size through a negative regulation of the transcription co-activator Yap1. The overexpression of hyperactive mutant Yap1 or deletion of key components in the Hippo pathway leads to increased organ size in different species. Analysis of interactions of this pathway with other cellular signals corroborating organ size control is limited in part due to the difficulties associated with development of rodent models.

Methods

Here, we develop a new model of reversible induction of the liver size in mice using siRNA-nanoparticles targeting two kinases of the Hippo pathway, namely, mammalian Ste20 family kinases 1 and 2 (Mst1 and Mst2), and an upstream regulator, neurofibromatosis type II (Nf2).

Results

The triple siRNAs nanop

SUBMITTER: Yin H 

PROVIDER: S-EPMC5381270 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature