Analyses of herpes simplex virus type 1 latency and reactivation at the single cell level using fluorescent reporter mice.

ABSTRACT: Herpes simplex virus type 1 (HSV-1) establishes a latent infection in sensory neurons from which the virus can periodically reactivate. Whilst latency establishment is thought to result from a failure to express immediate-early genes, we have previously shown that subpopulations of the latent neuronal reservoir have undergone lytic promoter activation prior to latency establishment. In the present study, we have investigated the biological properties of such latently infected neuronal subpopulations using Ai6 fluorescent reporter mice. Using this system we have determined that prior ICP0 or TK promoter activation does not correlate with increased latent virus DNA loads within individual cells and that neurons with evidence of historical lytic cycle promoter activity exhibit a comparable fr

SUBMITTER: Proenca JT 

PROVIDER: S-EPMC5381395 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature