Targeted Capture of Complete Coding Regions across Divergent Species.

ABSTRACT: Despite continued advances in sequencing technologies, there is a need for methods that can efficiently sequence large numbers of genes from diverse species. One approach to accomplish this is targeted capture (hybrid enrichment). While these methods are well established for genome resequencing projects, cross-species capture strategies are still being developed and generally focus on the capture of conserved regions, rather than complete coding regions from specific genes of interest. The resulting data is thus useful for phylogenetic studies, but the wealth of comparative data that could be used for evolutionary and functional studies is lost. Here, we design and implement a targeted capture method that enables recovery of complete coding regions across broad taxonomic scales. Capture pr

SUBMITTER: Schott RK 

PROVIDER: S-EPMC5381602 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature