Functional footprinting of regulatory DNA.

ABSTRACT: Regulatory regions harbor multiple transcription factor (TF) recognition sites; however, the contribution of individual sites to regulatory function remains challenging to define. We describe an approach that exploits the error-prone nature of genome editing-induced double-strand break repair to map functional elements within regulatory DNA at nucleotide resolution. We demonstrate the approach on a human erythroid enhancer, revealing single TF recognition sites that gate the majority of downstream regulatory function.

SUBMITTER: Vierstra J 

PROVIDER: S-EPMC5381659 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature