Development of an enzyme immunoassay for detection of antibodies against Coccidioides in dogs and other mammalian species.

ABSTRACT: Coccidioides is a soil-dwelling fungus that causes coccidioidomycosis, a disease also known as Valley fever, which affects humans and a variety of animal species. Recent findings of Coccidioides in new, unexpected areas of the United States have demonstrated the need for a better understanding of its geographic distribution. Large serological studies on animals could provide important information on the geographic distribution of this pathogen. To facilitate such studies, we used protein A/G, a recombinant protein that binds IgG antibodies from a variety of mammalian species, to develop an enzyme immunoassay (EIA) that detects IgG antibodies against Coccidioides in a highly sensitive and high-throughput manner. We showed the potential of this assay to be adapted to multiple animal species

SUBMITTER: Chow NA 

PROVIDER: S-EPMC5381914 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature