Humanized chondroitinase ABC sensitizes glioblastoma cells to temozolomide.

ABSTRACT:

Background

Malignant gliomas (glioblastomas; GBMs) are extremely aggressive and have a median survival of approximately 15 months. Current treatment modalities, which include surgical resection, radiation and chemotherapy, have done little to prolong the lives of GBM patients. Chondroitin sulfate proteoglycans (CSPG) are critical for cell-cell and cell-extracellular matrix (ECM) interactions and are implicated in glioma growth and invasion. Chondroitinase (Chase) ABC is a bacterial enzyme that cleaves chondroitin sulfate disaccharide chains from CSPGs in the tumor ECM. Wild-type Chase ABC has limited stability and/or activity in mammalian cells; therefore, we created a mutant humanized version (Chase M) with enhanced function in mammalian cells.

Methods

We hypothesized that

SUBMITTER: Jaime-Ramirez AC 

PROVIDER: S-EPMC5382089 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature