Phosphoproteomics of Primary Cells Reveals Druggable Kinase Signatures in Ovarian Cancer.

ABSTRACT: Our understanding of the molecular determinants of cancer is still inadequate because of cancer heterogeneity. Here, using epithelial ovarian cancer (EOC) as a model system, we analyzed a minute amount of patient-derived epithelial cells from either healthy or cancerous tissues by single-shot mass-spectrometry-based phosphoproteomics. Using a multi-disciplinary approach, we demonstrated that primary cells recapitulate tissue complexity and represent a valuable source of differentially expressed proteins and phosphorylation sites that discriminate cancer from healthy cells. Furthermore, we uncovered kinase signatures associated with EOC. In particular, CDK7 targets were characterized in both EOC primary cells and ovarian cancer cell lines. We showed that CDK7 controls cell proliferation and

SUBMITTER: Francavilla C 

PROVIDER: S-EPMC5382236 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature