The conserved protein Seb1 drives transcription termination by binding RNA polymerase II and nascent RNA.

ABSTRACT: Termination of RNA polymerase II (Pol II) transcription is an important step in the transcription cycle, which involves the dislodgement of polymerase from DNA, leading to release of a functional transcript. Recent studies have identified the key players required for this process and showed that a common feature of these proteins is a conserved domain that interacts with the phosphorylated C-terminus of Pol II (CTD-interacting domain, CID). However, the mechanism by which transcription termination is achieved is not understood. Using genome-wide methods, here we show that the fission yeast CID-protein Seb1 is essential for termination of protein-coding and non-coding genes through interaction with S2-phosphorylated Pol II and nascent RNA. Furthermore, we present the crystal structures of t

SUBMITTER: Wittmann S 

PROVIDER: S-EPMC5382271 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature