Quantitative 3D analysis of complex single border cell behaviors in coordinated collective cell migration.

ABSTRACT: Understanding the mechanisms of collective cell migration is crucial for cancer metastasis, wound healing and many developmental processes. Imaging a migrating cluster in vivo is feasible, but the quantification of individual cell behaviours remains challenging. We have developed an image analysis toolkit, CCMToolKit, to quantify the Drosophila border cell system. In addition to chaotic motion, previous studies reported that the migrating cells are able to migrate in a highly coordinated pattern. We quantify the rotating and running migration modes in 3D while also observing a range of intermediate behaviours. Running mode is driven by cluster external protrusions. Rotating mode is associated with cluster internal cell extensions that could not be easily characterized. Although the cluster

SUBMITTER: Cliffe A 

PROVIDER: S-EPMC5382290 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature