Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken lines.

ABSTRACT: Systems of antigen delivery into antigen-presenting cells represent an important novel strategy in chicken vaccine development. In this study, we verified the ability of Rous sarcoma virus (RSV) antigens fused with streptavidin to be targeted by specific biotinylated monoclonal antibody (anti-CD205) into dendritic cells and induce virus-specific protective immunity. The method was tested in four congenic lines of chickens that are either resistant or susceptible to the progressive growth of RSV-induced tumors. Our analyses confirmed that the biot-anti-CD205-SA-FITC complex was internalized by chicken splenocytes. In the cytokine expression profile, several significant differences were evident between RSV-challenged progressor and regressor chicken lines. A significant up-regulation of IL-2

SUBMITTER: Mucksova J 

PROVIDER: S-EPMC5382389 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature