Understanding CD8⁺ T-cell responses toward the native and alternate HLA-A*02:01-restricted WT1 epitope.

ABSTRACT: The Wilms' tumor 1 (WT1) antigen is expressed in solid and hematological malignancies, but not healthy tissues, making it a promising target for cancer immunotherapies. Immunodominant WT1 epitopes, the native HLA-A2/WT1_126-134 (RMFPNAPYL) (HLA-A2/RMFPNAPYL epitope (WT1A)) and its modified variant YMFPNAPYL (HLA-A2/YMFPNAPYL epitope (WT1B)), can induce WT1-specific CD8⁺ T cells, although WT1B is more stably bound to HLA-A*02:01. Here, to further determine the benefits of those two targets, we assessed the naive precursor frequencies; immunogenicity and cross-reactivity of CD8⁺ T cells directed toward these two WT1 epitopes. Ex vivo naive WT1A- and WT1B-specific CD8⁺ T cells were detected in healthy HLA-A*02:01⁺ indi

SUBMITTER: Nguyen TH 

PROVIDER: S-EPMC5382434 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature