Linkages between changes in the 3D organization of the genome and transcription during myotube differentiation in vitro.

ABSTRACT:

Background

The spatial organization of eukaryotic genomes facilitates and reflects the underlying nuclear processes that are occurring in the cell. As such, the spatial organization of a genome represents a window on the genome biology that enables analysis of the nuclear regulatory processes that contribute to mammalian development.

Methods

In this study, Hi-C and RNA-seq were used to capture the genome organization and transcriptome in mouse muscle progenitor cells (C2C12 myoblasts) before and after differentiation to myotubes, in the presence or absence of the cytidine analogue AraC.

Results

We observed significant local and global developmental changes despite high levels of correlation between the myotubes and myoblast genomes. Notably, the genes that exhibited t

SUBMITTER: Doynova MD 

PROVIDER: S-EPMC5382473 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature