Replication Protein A Prohibits Diffusion of the PCNA Sliding Clamp along Single-Stranded DNA.

ABSTRACT: The replicative polymerases cannot accommodate distortions to the native DNA sequence such as modifications (lesions) to the native template bases from exposure to reactive metabolites and environmental mutagens. Consequently, DNA synthesis on an afflicted template abruptly stops upon encountering these lesions, but the replication fork progresses onward, exposing long stretches of the damaged template before eventually stalling. Such arrests may be overcome by translesion DNA synthesis (TLS) in which specialized TLS polymerases bind to the resident proliferating cell nuclear antigen (PCNA) and replicate the damaged DNA. Hence, a critical aspect of TLS is maintaining PCNA at or near a blocked primer/template (P/T) junction upon uncoupling of fork progression from DNA synthesis by the repli

SUBMITTER: Hedglin M 

PROVIDER: S-EPMC5382571 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature