Preparation and Practical Applications of 2',7'-Dichlorodihydrofluorescein in Redox Assays.

ABSTRACT: Oxidative stress, a state in which intra- or extracellular oxidant production outweighs the antioxidative capacity, lies at the basis of many diseases. DCFH₂-DA (2',7'-dichlorodihydrofluorescein diacetate) is the most widely used fluorogenic probe for the detection of general oxidative stress. However, the use of DCFH₂-DA, as many other fluorogenic redox probes, is mainly confined to the detection of intracellular oxidative stress in vitro. To expand the applicability of the probe, an alkaline hydrolysis and solvent extraction procedure was developed to generate high-purity DCFH₂ (2',7'-dichlorodihydrofluorescein) from DCFH₂-DA using basic laboratory equipment. Next, the utility of DCFH₂ was exemplified in a variety of cell-free and in

SUBMITTER: Reiniers MJ 

PROVIDER: S-EPMC5382573 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature