Gene expression profiling reveals novel TGFbeta targets in adult lung fibroblasts.

ABSTRACT:

Background

Transforming growth factor beta (TGFbeta), a multifunctional cytokine, plays a crucial role in the accumulation of extracellular matrix components in lung fibrosis, where lung fibroblasts are considered to play a major role. Even though the effects of TGFbeta on the gene expression of several proteins have been investigated in several lung fibroblast cell lines, the global pattern of response to this cytokine in adult lung fibroblasts is still unknown.

Methods

We used Affymetrix oligonucleotide microarrays U95v2, containing approximately 12,000 human genes, to study the transcriptional profile in response to a four hour treatment with TGFbeta in control lung fibroblasts and in fibroblasts from patients with idiopathic and scleroderma-associated pulmonary fibrosis.

SUBMITTER: Renzoni EA 

PROVIDER: S-EPMC538264 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature