Interactions of the periplasmic binding protein CeuE with Fe(III) n-LICAM^4- siderophore analogues of varied linker length.

ABSTRACT: Bacteria use siderophores to mediate the transport of essential Fe(III) into the cell. In Campylobacter jejuni the periplasmic binding protein CeuE, an integral part of the Fe(III) transport system, has adapted to bind tetradentate siderophores using a His and a Tyr side chain to complete the Fe(III) coordination. A series of tetradentate siderophore mimics was synthesized in which the length of the linker between the two iron-binding catecholamide units was increased from four carbon atoms (4-LICAM^4-) to five, six and eight (5-, 6-, 8-LICAM^4-, respectively). Co-crystal structures with CeuE showed that the inter-planar angles between the iron-binding catecholamide units in the 5-, 6- and 8-LICAM^4- structures are very similar (111°, 110° and 110°) and allow

SUBMITTER: Wilde EJ 

PROVIDER: S-EPMC5382913 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature